Plasmid

Part:BBa_K5101004:Design

Designed by: Ziqi Mi   Group: iGEM24_ZQT-Nanjing   (2024-09-11)


Plasmid pET29(a)-pJ23119-SoxR-T-pSoxS-RBS-PhiX174E-T7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 572
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1009
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to ensure that the novel antimicrobial peptide of human origin can be successfully expressed in EcN, we performed codon optimization.


Source

This plasmid, pET29(a)-pJ23119-SoxR-T-pSoxS-RBS-PhiX174E-T7, utilizes the pET29a vector, commonly employed for robust gene expression in Escherichia coli. The components used in this design, including the J23119 promoter, SoxR/SoxS regulatory system, and the PhiX174E lysis gene, are all sourced from the iGEM Registry of Standard Biological Parts(BBa_J23119,BBa_K554003,BBa_K554000,BBa_K1835500). These parts have been selected for their proven functionality and compatibility in synthetic biology applications, facilitating the controlled expression and lysis within bacterial cells under specific conditions.

References